Article
Immunoreactivity of synthetic peptides derived from proteins of Cryptococcus gattii
Registro en:
BRANDÃO, Rafael Melo Santos de Serpa et al. Immunoreactivity of synthetic peptides derived from proteins of Cryptococcus gattii. Future Microbiology, v.9, n.7, p.871–878, 2014.
1746-0913
10.2217/FMB.14.49
1746-0921
Autor
Brandão, Rafael Melo Santos de Serpa
Martins, Liline Maria Soares
Andrade, Hélida Monteiro de
Faria, Angélica Rosa
Leal, Maria José Soares
Silva, Adalberto Socorro da
Wanke, Bodo
Lazéra, Márcia dos Santos
Vainstein, Marilene Henning
Mendes, Rinaldo Poncio
Moris, Daniela Vanessa
Cavalcante, Ricardo de Souza
Monte, Semiramis Jamil Hadad do
Resumen
This work was supported by the Laboratory of
Immunogenetics and Molecular Biology, Federal University of Piauí, Brazil. Aim: To determine the immunoreactivity of synthetic Cryptococcus-derived
peptides. Materials & methods: A total of 63 B-cell epitopes from previously identified
Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens
in ELISAs. The peptides were first evaluated for their ability to react against sera from
immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high
sensitivity and specificity in the first test were then retested with sera from individuals with
other fungal pathologies for cross-reactivity determination. Results: Six of 63 synthetic
peptides were recognized by antibodies in immunoassays, with a specificity of 100%,
sensitivity of 78% and low cross-reactivity. Conclusion: We successfully determined the
immunoreactivity of selected synthetic peptides of C. gattii derived proteins.