Article
Molecular detection of Leishmania braziliensis in Rattus norvegicus in an area endemic for cutaneous leishmaniasis in Brazil
Registro en:
MARCELINO, Andreza Pain et al. Molecular detection of Leishmania braziliensis in Rattus norvegicus in an area endemic for cutaneous leishmaniasis in Brazil. Vet Parasitol, v. 183, n. 1-2, p. 54-58, 2011
0304-4017
10.1016/j.vetpar.2011.06.019.
Autor
Marcelino, Andreza Pain
Ferreira, Eduardo de Castro
Avendanha, Jerônimo Sérgio
Costa, Cristiano F.
Chiarelli, Chiarelli
Almeida, Gregório
Moreira, Élvio Carlos
Leite, Romulo Cerqueira
Gontijo, Célia Maria Ferreira
Reis, Jenner Karlisson Pimenta dos
Resumen
Leishmania nested PCR (LnPCR) targeted to the SSUrRNA gene and DNA sequencing were used to analyze 315 tissue samples from 80 Rattus norvegicus specimens trapped in an area endemic for leishmaniasis in Belo Horizonte, Minas Gerais, Brazil. Of the samples analyzed, 17.46% (55/315) of all tissues, 10% (8/80) of skin, 26.92% (21/78) of blood, 30.76% (24/78) of bone marrow and 2.53% (2/79) of spleen were positive for Leishmania. The overall infection prevalence was 36.25% (29/80) The DNA sequencing showed that 65.51% (19/29) of the positive animals were infected by parasites belonging to theLeishmania braziliensis complex. The identification of L. braziliensis DNA in R. norvegicus in an area with a high prevalence of leishmaniasis might imply a zoonotic role of this species. The rodent control programs and health education may represent important measures toward the control of leishmaniasis.