Article

A preliminary molecular epidemiologic study using analysis of variable number of tandem repeats of Acinetobacter baumannii OXA-23 producing strains isolated from hospitals in Rio de Janeiro State, Brazil

Estudo epidemiológico molecular preliminar usando a análise de número variável de repetições em tandem (VNTR) de cepas de Acinetobacter baumannii produtoras de OXA-23 isoladas em hospitais no Rio de Janeiro, Brasil;
Estudio epidemiológico molecular preliminar usando el análisis de número variable en tándem de repeticiones (VNTR) de cepas de Acinetobacter baumannii productoras de OXA-23 aisladas en hospitales de Rio de Janeiro, Brasil

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CARRAMACHI, Isabel Nogueira et al. A preliminary molecular epidemiologic study using analysis of variable number of tandem repeats of Acinetobacter baumannii OXA-23 producing strains isolated from hospitals in Rio de Janeiro State, Brazil. Rev Pan-Amaz Saude, v.5, n.2, Ananindeua jun. 2014
2176-6223
10.5123/S2176-62232014000200008
Author
Carramachi, Isabel Nogueira
Carvalho, Karyne Rangel
Cruz, Jéssica Gonçalves Vieira da
Zahner, Viviane
Institutions
Abstract
The Gram-negative multidrug-resistant (MDR) bacterium Acinetobacter baumannii constitutes a serious cause of nosocomial infections in Brazilian hospitals. A panel of 36 strains, belonging to five pre-determined pulsed-field gel electrophoresis (PFGE) genotypes from four different hospitals in the City of Rio de Janeiro, Rio de Janeiro State, Brazil, was submitted to simple agarose gel electrophoresis to determine the variation of nine variable number tandem repeats (VNTR) band profiles. Based on published data VNTR were classified in two categories, long (L) and short (S) repeats with different evolutionary implications. The results demonstrated the superior discrimination of VNTR over PFGE. The VNTR Abaum_3002 marker was the least discriminatory with only one allele, while VNTR10 and Abaum_2240 each presented four alleles. The use of a combination of the nine VNTR resulted in a refined genotyping tool that provides valuable epidemiological information.
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