Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener

dc.creatorFerrari, I.
dc.creatorLorenzi, H.
dc.creatorSantos, M. R.
dc.creatorBrandariz, S.
dc.creatorRquena, J. M.
dc.creatorSchijman, A.
dc.creatorVázquez, M.
dc.creatorSilveira, J. F. da
dc.creatorBen-Dov, C.
dc.creatorMedrano, C.
dc.creatorGhio, S.
dc.creatorBergami, P. López
dc.creatorCano, I.
dc.creatorZingalez, B.
dc.creatorUrmenyi, T. P.
dc.creatorRondinelli, E.
dc.creatorGonzález, A.
dc.creatorCortes, A.
dc.creatorLopez, M. C.
dc.creatorThomaz, M. C.
dc.creatorAlonso, C.
dc.creatorRamírez, J. L.
dc.creatorChiurrillo, M. A.
dc.creatorAldao, R. Rangel
dc.creatorBrandão, Adeilton
dc.creatorDegrave, Wim
dc.creatorPerrot, V.
dc.creatorSaumier, M.
dc.creatorBillaut, A.
dc.creatorCohen, D.
dc.creatorLe Paslier, D.
dc.creatorLevin, M. J.
dc.date2020-10-09T15:07:19Z
dc.date2020-10-09T15:07:19Z
dc.date1997
dc.date.accessioned2023-09-26T20:29:14Z
dc.date.available2023-09-26T20:29:14Z
dc.identifierFERRARI, I. et al. Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 843-852, Nov./Dec. 1997.
dc.identifier0074-0206
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43903
dc.identifier10.1590/S0074-02761997000600021
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857980
dc.descriptionStrategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CLBrener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectProjeto genoma
dc.subjectMapa físico
dc.subjectBiblioteca YAC
dc.subjectBiblioteca BAC
dc.subjectLocais marcados por sequência (STS)
dc.subjectTags de sequência expressas (EST)
dc.subjectTrypanosoma cruzi
dc.subjectGenome project
dc.subjectPhysical map
dc.subjectYAC library
dc.subjectBAC library
dc.subjectSequence tagged sites (STS)
dc.subjectExpressed sequence tags (EST)
dc.titleTowards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener
dc.typeArticle


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