Article
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil
Registro en:
GÓMEZ, Mariela M. et al. Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil. Infection, Genetics and Evolution, v.28, p.385–388, dec. 2014.
1567-1348
Autor
Gómez, Mariela Martínez
Resque, Hugo Reis
Volotão, Eduardo de Mello
Rose, Tatiana Lundgren
Silva, Marcelle Fiqueira Marques da
Heylen, Elisabeth
Zeller, Mark
Matthijnssens, Jelle
Leite, José Paulo Gagliardi
Resumen
G12 group A rotavirus (RVA) are currently recognized as a globally emerging genotype and have been
described in combination with several P-types. In Brazil, G12 RVA strains have been described in the
Southern (2003) and Northern (2008–2010) regions, in combination with the P[9] and P[6] genotype,
respectively. To date, few complete genomes of G12 RVA strains have been described (none from Brazilian
strains), considering G12P[9] genotype just one strain, RVA/Human-tc/THA/T152/1998/G12P[9], has
their 11 gene segments characterized. This study aims to determine the genomic constellation of G12P[9]
and G12P[8] RVA strains detected in Brazil between 2006 and 2011. Therefore, the eleven gene segments
of five Brazilian G12 RVA strains were amplified and sequenced, and the genotype of each gene segment
was assigned using phylogenetic analysis. Complete genome analyses of G12 RVA strain circulating
between 2006 and 2011 in Brazil revealed a conserved Wa-like genomic constellation for three
G12P[8] RVA strains; whereas the two G12P[9] strains possessed distinct reassorted AU-1-like genomic
constellations, closely related to the reference strain RVA/Human-tc/THA/T152/1998/G12P[9] in most
genes. The results obtained in the current study suggest that G12P[9] (AU-1-like) and G12P[8] (Wa-like)
strains detected in different regions of Brazil do not share a common origin. Moreover, while Brazilian
G12P[8] RVA strains showed a complete Wa-like human constellation, both G12P[9] strains possessed
an NSP1 gene of bovine origin (NSP1), and RVA/Human-wt/BRA/PE18974/2010/G12P[9] also possessed
a VP3 gene of canine/feline origin.