Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.

dc.creatorVidal, Daniel Torres Andion
dc.creatorFortunato, Gustavo
dc.creatorKlein, Wilfried
dc.creatorCortizo, L
dc.creatorVasconcelos, Juliana Fraga
dc.creatorSantos, Ricardo Ribeiro dos
dc.creatorSoares, Milena Botelho Pereira
dc.creatorMacambira, Simone Garcia
dc.date2014-10-10T16:54:22Z
dc.date2014-10-10T16:54:22Z
dc.date2012
dc.date.accessioned2023-09-26T20:27:42Z
dc.date.available2023-09-26T20:27:42Z
dc.identifierVIDAL, D. T. A. et al. Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances. Revista Portuguesa de Pneumologia, v. 18, n. 3, p. 128-136, 2012.
dc.identifier0873-2159
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8575
dc.identifier10.1016/j.rppneu.2011.12.007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857429
dc.descriptionSeveral experimental studies of pulmonary emphysema using animal models have been described in the literature. However, only a few of these studies have focused on the assessment of ergometric function as a non-invasive technique to validate the methodology used for induction of experimental emphysema. Additionally, functional assessments of emphysema are rarely correlated with morphological pulmonary abnormalities caused by induced emphysema. The present study aimed to evaluate the effects of elastase administered by tracheal puncture on pulmonary parenchyma and their corresponding functional impairment. This was evaluated by measuring exercise capacity in C57Bl/6 mice in order to establish a reproducible and safe methodology of inducing experimental emphysema. Thirty six mice underwent ergometric tests before and 28 days after elastase administration. Pancreatic porcine elastase solution was administered by tracheal puncture, which resulted in a significantly decreased exercise capacity, shown by a shorter distance run (-30.5%) and a lower mean velocity (-15%), as well as in failure to increase the elimination of carbon dioxide. The mean linear intercept increased significantly by 50% in tracheal elastase administration. In conclusion, application of elastase by tracheal function in C57Bl/6 induces emphysema, as validated by morphometric analyses, and resulted in a significantly lower exercise capacity, while resulting in a low mortality rate.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier España
dc.rightsopen access
dc.subjectPulmonary empysema
dc.subjectErgometric test
dc.subjectPorcine elastase
dc.subjectC57Bl/6
dc.subjectEnfisema Pulmonar
dc.subjectModelos Animais de Doenças
dc.subjectPulmão/efeitos de drogas
dc.subjectPulmão/patologia
dc.subjectElastase Pancreática/farmacologia
dc.subjectAnimais
dc.subjectFeminino
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos C57BL
dc.subjectEnfisema Pulmonar/induzido quimicamente
dc.titleAlterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
dc.typeArticle


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