Article
Leishmanicidal and antitumoral activities of endophytic fungi associated with the Antarctic angiosperms Deschampsia antarctica Desv. and Colobanthus quitensis (Kunth) Bartl.
Registro en:
SANTIAGO, Iara Furtado et al. Leishmanicidal and antitumoral activities of endophytic fungi associated with the Antarctic angiosperms Deschampsia antarctica Desv. and Colobanthus quitensis (Kunth) Bartl. Extremophiles. 2012, vol.16, n., 1, pp. 95-103
1431-0651
10.1007/s00792-011-0409-9
Autor
Santiago, Iara Furtado
Alves, Tania Maria Almeida
Rabello, Ana Lucia Teles
Sales Junior, Policarpo Ademar
Romanha, Alvaro Jose
Zani, Carlos Leomar
Rosa, Carlos Augusto
Rosa, Luiz Henrique
Resumen
A total of 564 isolates of endophytic fungi were recovered from the plants Deschampsia antarctica and Colobanthus quitensis collected from Antarctica. The isolates were screened against parasites Leishmania amazonensis and Trypanosoma cruzi and against the human tumour cell lines. Of the 313 fungal isolates obtained from D. antarctica and 251 from C. quitensis, 25 displayed biological activity. Nineteen extracts displayed leishmanicidal activity, and six inhibited the growth of at least one tumour cell line. These fungi belong to 19 taxa of the genera Alternaria, Antarctomyces, Cadophora, Davidiella, Helgardia, Herpotrichia, Microdochium, Oculimacula, Phaeosphaeria and one unidentified fungus. Extracts of 12 fungal isolates inhibited the proliferation of L. amazonesis at a low IC50 of between 0.2 and 12.5 lg ml-1 . The fungus Phaeosphaeria herpotrichoides displayed only leishmanicidal activity with an IC50 of 0.2 lg ml-1 , which is equivalent to the inhibitory value of amphotericin B. The extract of Microdochium phragmitis displayed specific cytotoxic activity against the UACC-62 cell line with an IC50 value of 12.5 lg ml-1 . Our results indicate that the unique angiosperms living in Antarctica shelter an interesting bioactive fungal community that is able to produce antiprotozoal and antitumoral molecules. These molecules may be used to develop new leishmanicidal and anticancer drugs