Species distribution, sequence types and antimicrobial resistance of Acinetobacter spp. from cystic fibrosis patients

dc.creatorRocha, G. A.
dc.creatorLima, D. F.
dc.creatorRodrigues, E. R.
dc.creatorLeão, R. S.
dc.creatorFolescu, T. W.
dc.creatorFirmida, M. C.
dc.creatorCohen, R. W. F.
dc.creatorAlbano, R. M.
dc.creatorMarques, E. A.
dc.date2020-04-14T17:55:18Z
dc.date2020-04-14T17:55:18Z
dc.date2018
dc.date.accessioned2023-09-26T20:22:30Z
dc.date.available2023-09-26T20:22:30Z
dc.identifierRocha, G. A., Lima, D. F., Rodrigues, E. R., Leão, R. S., Folescu, T. W., Firmida, M. C., … Marques, E. A. (2018). Species distribution, sequence types and antimicrobial resistance of Acinetobacter spp. from cystic fibrosis patients. Epidemiology and Infection, 146(4), 524–530. doi: 10.1017/S0950268817002849
dc.identifier0950-2688
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/40753
dc.identifier10.1017/S0950268817002849
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8855463
dc.descriptionAcinetobacter spp. são patógenos importantes da saúde, estando intimamente ligados à resistência a antibióticos e surtos em todo o mundo. Embora essas espécies raramente sejam observadas em pacientes com fibrose cística (FC), descrevemos as características de 53 cepas de Acinetobacter spp. isolado do escarro de 39 pacientes brasileiros com FC. A distribuição das espécies foi A. baumannii (n = 29), A. pittii (n = 13), A. nosocomialis (n = 8), A. seifertii (n = 1), A. soli (n = 1) e A variabilis (n = 1) determinada por seqüenciamento parcial do gene rpoB. Dezesseis cepas (10 A. baumannii, 3 A. pittii e 3 A. nosocomialis) foram multirresistentes (MDR) pelo teste de difusão em disco (30%) e oito cepas de A. baumannii resistentes ao carbapenêmico MDR abrigavam o bla OXA-23- como o gene da oxacilinase. Trinta e três tipos de sequência (STs) foram identificados por tipagem de seqüência multilocus, dos quais oito eram novos (A. baumannii: 843, 844, 845, 847, 848; A. pitti: 643; A. nosocomialis: 862 e A. seifertii: 846); seis STs (2 A. baumannii, 3 A. pittii e 1 A. nosocomialis) foram encontrados em mais de um paciente. Quatro linhagens de A. baumannii foram atribuídas a dois complexos clonais comuns (CCs), a saber, CC1 (ST1, ST20 e ST160) e CC79 (ST79). Este estudo destaca a extensa diversidade de espécies de Acinetobacter spp. cepas nas infecções pulmonares por FC que podem apresentar dificuldades para o tratamento devido à resistência antimicrobiana significativa.
dc.descriptionAcinetobacter spp. are important healthcare pathogens, being closely linked to antibiotic resistance and outbreaks worldwide. Although such species are rarely observed in patients with cystic fibrosis (CF), we describe the characteristics of 53 strains of Acinetobacter spp. isolated from the sputum of 39 Brazilian patients with CF. The species distribution was A. baumannii (n = 29), A. pittii (n = 13), A. nosocomialis (n = 8), A. seifertii (n = 1), A. soli (n = 1) and A. variabilis (n = 1) determined by partial rpoB gene sequencing. Sixteen strains (10 A. baumannii, 3 A. pittii and 3 A. nosocomialis) were multidrug-resistant (MDR) by disk diffusion test (30%) and eight MDR carbapenem-resistant A. baumannii strains harboured the bla OXA-23-like oxacillinase gene. Thirty-three sequence types (STs) were identified by multilocus sequence typing of which eight were novel (A. baumannii: 843, 844, 845, 847, 848; A. pitti: 643; A. nosocomialis: 862 and A. seifertii: 846); six STs (2 A. baumannii, 3 A. pittii and 1 A. nosocomialis) were found in more than one patient. Four strains of A. baumannii were assigned to two common clonal complexes (CCs), namely, CC1 (ST1, ST20 and ST160), and CC79 (ST79). This study underlines the extensive species diversity of Acinetobacter spp. strains in CF lung infections which may present difficulties for therapy due to significant antimicrobial resistance.
dc.description2200-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherCambridge University Press
dc.rightsrestricted access
dc.subjectAcinetobacter Infections/drug therapy
dc.subjectSputum/microbiology
dc.subjectRetrospective Studies
dc.subjectPolymerase Chain Reaction
dc.subjectMicrobial Sensitivity Tests
dc.subjectHumans
dc.subjectDrug Resistance, Bacterial
dc.subjectCystic Fibrosis/microbiology
dc.subjectChild
dc.subjectBrazil/epidemiology
dc.subjectAcinetobacter Infections/microbiology
dc.subjectAcinetobacter Infections/epidemiology
dc.subjectAdult
dc.subjectAnti-Bacterial Agents/pharmacology
dc.subjectAlgorithms
dc.subjectAcinetobacter spp .
dc.subjectFibrose cística
dc.subjectResistência a múltiplas drogas
dc.subjectDigitação de sequência multilocus
dc.subjectAcinetobacter spp.
dc.subjectCystic fibrosis
dc.subjectMultidrug resistance
dc.subjectMultilocus sequence typing
dc.titleSpecies distribution, sequence types and antimicrobial resistance of Acinetobacter spp. from cystic fibrosis patients
dc.typeArticle


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