Article
Hydration and Conformational Equilibrium in Yeast Thioredoxin 1: Implication for H+ Exchange
Registro en:
SILVA, Carolina Cruzeiro et al. Hydration and Conformational Equilibrium in Yeast Thioredoxin 1: Implication for H+ Exchange. Biochemistry, v.53, n.18, p.2890–2902, 2014.
0006-2960
10.1021/bi401542v
Autor
Silva, Carolina Cruzeiro
Gomes Neto, Francisco
Machado, Luciana E. S. F.
Myamoto, Catarina A.
Pinheiro, Anderson S.
Pereira, Natalia Correa
Magalhães, Mariana T. Q. de
Valente, Ana Paula
Almeida, Fabio C. L.
Resumen
One of the ancestral features of thioredoxins is the presence of a water cavity. Here, we report that a largely hydrated, conserved, buried aspartic acid in the water cavity modulates the dynamics of the interacting loops of yeast thioredoxin 1 (yTrx1). It is well-established that the aspartic acid, Asp24 for yTrx1, works as a proton acceptor in the reduction of the target protein. We propose a complementary role for Asp24 of coupling hydration and conformational motion of the water cavity and interacting loops. The intimate contact between the water cavity and the interacting loops means that motion at the water cavity will affect the interacting loops and vice versa. The D24N mutation alters the conformational equilibrium for both the oxidized and reduced states, quenching the conformational motion in the water cavity. By measuring the hydration and molecular dynamics simulation of wild-type yTrx1 and the D24N mutant, we showed that Asn24 is more exposed to water than Asp24 and the water cavity is smaller in the mutant, closing the inner part of the water cavity. We discuss how the conformational equilibrium contributes to the mechanism of catalysis and H+ exchange.