Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations

dc.creatorSouza, Raquel Santos
dc.creatorDiaz-Albiter, Hector Manuel
dc.creatorDillon, Vivian Maureen
dc.creatorDillon, Rod J.
dc.creatorGenta, Fernando Ariel
dc.date2016-12-08T16:47:07Z
dc.date2016-12-08T16:47:07Z
dc.date2016
dc.date.accessioned2023-09-26T20:22:18Z
dc.date.available2023-09-26T20:22:18Z
dc.identifierSOUZA, Raquel Santos; et al. Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations. Plos One, v.11, n.3, e0151403, 16p, Mar. 2016
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16492
dc.identifier10.1371/journal.pone.0151403
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8855378
dc.descriptionAedes aegypti larvae ingest several kinds of microorganisms. In spite of studies regarding mosquito digestion, little is known about the nutritional utilization of ingested cells by larvae. We investigated the effects of using yeasts as the sole nutrient source for A. aegypti larvae. We also assessed the role of beta-1,3-glucanases in digestion of live yeast cells. Beta-1,3-glucanases are enzymes which hydrolyze the cell wall beta-1,3-glucan polyssacharide. Larvae were fed with cat food (controls), live or autoclaved Saccharomyces cerevisiae cells and larval weight, time for pupation and adult emergence, larval and pupal mortality were measured. The presence of S. cerevisiae cells inside the larval gut was demonstrated by light microscopy. Beta-1,3-glucanase was measured in dissected larval samples. Viability assays were performed with live yeast cells and larval gut homogenates, with or without addition of competing beta-1,3-glucan. A. aegypti larvae fed with yeast cells were heavier at the 4th instar and showed complete development with normal mortality rates. Yeast cells were efficiently ingested by larvae and quickly killed (10% death in 2 h, 100% in 48 h). Larvae showed beta-1,3-glucanase in head, gut and rest of body. Gut beta-1,3-glucanase was not derived from ingested yeast cells. Gut and rest of body activity was not affected by the yeast diet, but head homogenates showed a lower activity in animals fed with autoclaved S. cerevisiae cells. The enzymatic lysis of live S. cerevisiae cells was demonstrated using gut homogenates, and this activity was abolished when excess beta-1,3-glucan was added to assays. These results show that live yeast cells are efficiently ingested and hydrolyzed by A. aegypti larvae, which are able to fully-develop on a diet based exclusively on these organisms. Beta-1,3-glucanase seems to be essential for yeast lytic activity of A. aegypti larvae, which possess significant amounts of these enzyme in all parts investigated.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectAedes
dc.subjectLarvas de mosquito
dc.subjectProteínas de Saccharomyces cerevisiae
dc.subjectConsiderações fisiológicas e bioquímicas
dc.subjectAedes aegypti
dc.subjectMosquito Larvae
dc.subjectYeasts
dc.subjectBeta-1,3- glucanases
dc.subjectSaccharomyces cerevisiae
dc.subjectPhysiological and Biochemical Considerations
dc.titleDigestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations
dc.typeArticle


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