Diagnosis of Streptococcus pneumoniae meningitis by polymerase chain reaction amplification of the gene for pneumolysin

dc.creatorMatos, Juliana de A.
dc.creatorMadureira, Danielle J.
dc.creatorRebelo, Maria C.
dc.creatorHofer, Cristina B.
dc.creatorBarroso, David E.
dc.date2020-01-23T11:25:09Z
dc.date2020-01-23T11:25:09Z
dc.date2006
dc.date.accessioned2023-09-26T20:20:49Z
dc.date.available2023-09-26T20:20:49Z
dc.identifierMATOS, Juliana de A. et al. Diagnosis of Streptococcus pneumoniae meningitis by polymerase chain reaction amplification of the gene for pneumolysin. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 5, p. 559-563, Aug. 2006.
dc.identifier0074-0206
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/39446
dc.identifier10.1590/s0074-02762006000500014
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8854788
dc.descriptionDiagnosis of bacterial meningitis has long been based on classical methods of Gram stain, serological tests, and culture of cerebrospinal fluid (CSF). The performance of these methods, especially culture and direct smear is thwarted by failure to detect bacteria following administration of antimicrobial agents and reluctance to performance lumbar punctures at admission. Indeed, patients with meningitis frequently receive antibiotics orally or by injection before the diagnosis is suspected or established Thus an alternative method has become necessary to help clinicians and epidemiologists to management and control of bacterial meningitis. We evaluate the application of a polymerase chain reaction-based (PCR) assay for amplification of pneumolysin gene (ply) to diagnosis of Streptococcus pneumoniae meningitis. The PCR assay sensitivity for CSF was 96% (95% confidence interval, CI, 90-99%) compared to a sensitivity of 59% for culture (95% CI 49-69%), 66% for Gram stain (95% CI 56-74%), and 78% for latex agglutination test (95% CI 69-86%); PCR specificity was 100% (95% CI 83-100%). PCR results were available within 4 h of the start of the assay. This molecular approach proved to be reliable and useful to identify this bacterium compared with other classical laboratory methods for identification of bacterial meningitis pathogens.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectStreptococcus pneumoniae
dc.subjectMeningite
dc.subjectReação em cadeia da polimerase
dc.subjectStreptococcus pneumoniae
dc.subjectMeningitis
dc.subjectPolymerase chain reaction
dc.subjectPneumolysin ply gene
dc.titleDiagnosis of Streptococcus pneumoniae meningitis by polymerase chain reaction amplification of the gene for pneumolysin
dc.typeArticle


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