Article
CD8+ T cells in situ in different clinical forms of human cutaneous leishmaniasis.
Registro en:
DANTAS, M. L. et al. CD8+ T cells in situ in different clinical forms of human cutaneous leishmaniasis. Revista da Sociedade Brasileira de Medicina Tropical, v. 46, n. 6, p. 728-734, 2013.
1678-9849
dx.doi.org/10.1590/0037-8682-0174-2013
Autor
Dantas, Marina Loyola
Oliveira, Juliana Cabral de
Carvalho, Lucas Pedreira de
Passos, Sara Timóteo
Queiroz, Adriano
Machado, Paulo Roberto
Carvalho Filho, Edgar Marcelino
Arruda, Sérgio Marcos
Resumen
Introduction: Leishmania braziliensis infection induces a large spectrum of lesions that clinically manifest as nodules or papules
that progress to ulcers. Although it is already known that T helper cells predominate in the lesions, cytotoxic T cells have also
been reported to be present, and their role in leishmaniasis immunopathogenesis is not well known. This study investigated
the amounts of CD8+ and granzyme B+ cells in different clinical forms of human cutaneous leishmaniasis (CL). Methods:
Forty tissue fragments from early (E-CL) and late CL (L-CL) lesions and from disseminated leishmaniasis (DL) - papules and
ulcers - were characterized. The infl amed area per fragment was calculated, and the CD8 and granzyme B expression levels
in the infi ltrates were quantifi ed by counting positive cells in 15 fi elds. The localization of CD8 and granzyme B was graded
subjectively. Results: Infl ammation was higher in L-CL and DL ulcers. CD8 expression was increased in late ulcerated lesions
compared to recent lesions. The increase in CD8+ cells also correlated with the duration of the lesion. Papules had a higher
frequency of granzyme B+ cells than E-CL lesions, although the frequency was similar to those for late and DL ulcers. CD8+
cells were mostly found in the papillary dermis. Conclusions: CD8+ T and granzyme B+ cells are present in the infl ammatory
infi ltrates of CL and DL and may participate in the immunopathogenesis of Leishmania infection.