Characterisation of Neisseria meningitidis cc11/ET-15 variant by whole genome sequencing

dc.creatorSantos, Debora Ribeiro de Souza
dc.creatorBianco, Kayo
dc.creatorClementino, Maysa Beatriz Mandetta
dc.creatorDávila, Alberto Martín Rivera
dc.creatorFilippis, Ivano de
dc.date2022-11-30T19:01:24Z
dc.date2022-11-30T19:01:24Z
dc.date2022
dc.date.accessioned2023-09-26T20:18:12Z
dc.date.available2023-09-26T20:18:12Z
dc.identifierSANTOS, Debora Ribeiro de Souza et al. Characterisation of Neisseria meningitidis cc11/ET-15 variant by whole genome sequencing. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 117, e220118. p. 1 - 10, Sep. 2022.
dc.identifier0074-0206
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/55844
dc.identifier10.1590/0074-02760220118
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8853752
dc.descriptionBACKGROUND Neisseria meningitidis strains belonging to clonal complex 11 is the cause of numerous outbreaks and epidemics in the United States, Canada and Europe, accounting for 49.5% of cases of meningococcal disease caused by serogroup C worldwide. In Brazil, it is the second most frequent clonal complex within this serogroup. The genetic characterisation of cc11/ ET-15 variants is important for the epidemiological monitoring of meningococcal disease, through the identification of circulating epidemic clones, to support specific actions of Health Surveillance aiming outbreaks control. OBJECTIVES The objective of this study was to identify features in the genome of cc11/ET-15 clones through whole-genome sequencing (WGS), that differ from cc11/non-ET-15 strains that could explain their virulence. METHODS The whole genome of three cc11/ET-15 representative strains were sequenced with a minimum coverage of 100X with the MiSeq System and compared to the genome of cc11/non-ET-15 strains. RESULTS Genome analysis of cc11/ET-15 variants showed the presence of resistance factors, mobile genetic elements and virulence factors not found in cc11/non-ET-15 strains. MAIN CONCLUSIONS Our results show that these strains carry virulence factors not identified in cc11/non-ET-15 strains, which could explain the high lethality rates attributed to this clone worldwide.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectSequenciamento de genoma completo (WGS)
dc.subjectNeisseria meningitidis cc11/ET-15
dc.subjectWhole-genome sequencing (WGS)
dc.subjectNeisseria meningitidis cc11/ET-15
dc.titleCharacterisation of Neisseria meningitidis cc11/ET-15 variant by whole genome sequencing
dc.typeArticle


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