Article
Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion
Registro en:
DE OLIVEIRA SILVA, F. et al. Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion. Cell and Tissue Research, v. 346, n. 2, p. 237–244, nov. 2011.
1432-0878
10.1007/s00441-011-1239-x
Autor
Silva, Flávio de Oliveira
Santos, Priscila das Neves
Melo, Cristiane Moutinho Lagos de
Teixeira, Edson Holanda
Cavada, Benildo de Sousa
Arruda, Francisco Vassiliepe Sousa
Cajazeiras, João Batista
Almeida, Alysson Chaves
Pereira, Valéria Alves Rêgo
Porto, Ana Lúcia Figueiredo
Resumen
Fundação de Amparo à Pesquisa de Pessoal de Nível Superior da CAPES (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco, processo APQ-0493-2.11 / 08) e do Centro de Pesquisas Aggeu Magalhães. Fundação Oswaldo Cruz em Recife, Brasil (CPqAM / FIOCRUZ). Lectins constitute a class of glycoproteins, which are capable of selectively and reversibly binding to carbohydrates, distinguishing small structural differences in complex oligosaccharides. Studies have shown that the binding of lectins to cell-surface carbohydrates can lead to various effects such as cellular proliferation, histamine release and cytokine production. Canavalia brasiliensis lectin (ConBr) is a (D-mannose) D-glucose lectin. In this study, murine splenocytes were cultured to determine the effect of ConBr on cell proliferation, nitric oxide (NO) release and cytokine secretion. In addition, cellular viability assays were performed to evaluate any mitogenic activity induced by this lectin. ConBr significantly increased cell proliferation with minimal cell damage. This lectin was able to induce an increased production of cytokines such as IL-2, IL-6 and IFN-γ and a decreased production of IL- 10. The release of NO was also observed. The results of this study indicate that ConBr could potentially be used as an immunomodulator. 2050-01-01