Article
The inability of a dengue NS1 ELISA to detect Zika infections
Registro en:
LIMA, Monique da Rocha Queiroz et al. The inability of a dengue NS1 ELISA to detect Zika infections. Scientifc Reports, v. 9, p. 1-7, 2019.
2045-2322
10.1038/s41598-019-55160-4
Autor
Lima, Monique da Rocha Queiroz
Carneiro, Thais Chouin
Azeredo, Elzinandes
Barbosa, Luciana Santos
Souza, Thiara Manuele Alves
Silva, Jéssica Badolato Corrêa da
Nunes, Priscila Conrado Guerra
Dal Fabbro, Márcia
Facco, Izilyanne H. Romanholi
Cunha, Rivaldo Venâncio da
Santos, Flavia Barreto dos
Resumen
The presence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) in Brazil, may result in a difficult diagnosis due to the signs and symptoms shared by those. Moreover, as DENV and ZIKV belong to the same family, serological assays may show a high rate of cross-reactivity. Here, we evaluated a Dengue NS1 capture assay for early and differential diagnosis of dengue during the Zika epidemic occurred in Brazil in 2016. Samples (n = 227) from 218 patients included sera, plasma and urine from previously confirmed acute cases of Zika, dengue and Zika/dengue co-infections. Nine of those patients presented two specimens. The Dengue NS1 test was very specific for dengue diagnosis (99.32%), even in the co-circulation with ZIKV, and exhibited a high accuracy in not detecting acute Zika infections (92.43%). Our findings showed that the dengue NS1 capture test analyzed here was not able to recognize the ZIKV NS1 and its potential for cross-reaction.