Article
Metallothionein determination can be applied to learn about aquatic metal pollution and oxidative stress detoxification mechanisms through Problem-based Learning
Registro en:
HAUSER-DAVIS, Rachel; COMARÚ, Michele W.; LOPES, Renato M. Metallothionein determination can be applied to learn about aquatic metal pollution and oxidative stress detoxification mechanisms through Problem-based Learning. Biochem Mol Biol Educ., v. 48, p. 291-296, 2020.
1539-3429
10.1002/bmb.21342
Autor
Hauser-Davis, Rachel A.
Comaru, Michele W.
Lopes, Renato M.
Resumen
A simple, repeatable and inexpensive laboratory practice applied to teach and
discuss aquatic metal pollution and oxidative stress detoxification mechanisms
through biomarker analyses, as well as important ecotoxicology concepts, is
presented herein. It has been implemented in a university in Brazil to both
undergraduate and Master's and PhD students, indicating usefulness to all
these levels. Students learned to detect metallothionein and reduced glutathione concentrations in biological samples and investigate several variables of
interest in biomonitoring assessments. In addition, statistical correlations were
used to indicate the potential dual role played by MT in aquatic organisms, allowing for biological inferences regarding both aquatic metal pollution and oxidative stress detoxification mechanisms and maturing of ecotoxicological and
biomonitoring concepts discussed and presented both theoretically and integrated to the laboratory findings. 2022-01-01