Article
Photodynamic antimicrobial chemotherapy (PACT) using Phenothiazines Derivatives Associated with the Red Laser against Staphylococcus aureus
Registro en:
OLIVEIRA, S. C. P. D. et al. Photodynamic antimicrobial chemotherapy (PACT) using Phenothiazines Derivatives Associated with the Red Laser against Staphylococcus aureus. Proceedings of SPIE, v. 8569, p. 1-6, 2013.
0277-786X
10.1117/12.2005560
Autor
Oliveira, Susana Carla Pires Sampaio
Santos, Gustavo Miranda Pires
Monteiro, Juliana Santos de Carvalho
Miranda, Anderson Fontes Suzart
Sampaio, Fernando José Pires
Gesteira, Maria de Fatima Malvar
Zanin, Fátima A. A
Santos, Marcos André Vannier dos
Pinheiro, Antonio Luiz Barbosa
Resumen
The objective of this study was to evaluate the bactericidal effect of photodynamic antimicrobial chemotherapy (PACT) using phenothiazinium dye (Toluidine blue O and methylene blue) at a low concentration of 1μg/mL irradiated with the red laser at doses of 2.4 e 4.8 J/cm² on strain of Staphylococcus aureus (ATCC 23529) in vitro. For this research, tests
were performed in triplicate and the samples were distributed into six test groups: (L-P-) Negative control (L1+P-) and (L2+P-) bacterial suspensions were irradiated with laser energy 2.4 and 4.8 J/cm2 respectively in the absence of photosensitizer; (L1+P+) and (L2+P+) bacterial suspensions were irradiated with laser in the presence of 1μg/ml of photosensitizer and finally (L-P+) bacterial suspensions only in the presence of phenothiazinium dye. Therefore, were analyzed the potential bactericidal PACT by counting of colony-forming units and analyzed statistically (ANOVA, Tukey test, p<0.05). The results showed that the negative control group when compared with laser group (L2+P-) it was
observed a statistically significant increase (p<0.01) which L2+P- showed a higher number of CFU, on the other hand when compared to L1+P- no statistically significant difference was found, relation to the groups submitted to PACT, only showed a statistically significant reduction relative to the group irradiated L2+P+ (p<0.01) that showed a decrease in the number of CFU. There was no statistically significant difference between the groups submitted to PDT (L1+P+ and L2+P+). Although the results of this study have shown a reduction in average number of colony forming units by the appropriate laser-dye treatment combination, it needs further investigation.