Article
Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil
Registro en:
CORRÊA, Thais G. et al. Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil. Marine Biodiversity Records, v.6, e30, 4p, 2013.
1755-267
Autor
Sholl, Thais G. Corrrêa
Moura, Jailson Fulgêncio de
Ott, Paulo Henrique
Bonvicino, Cibele R.
Reis, Estéfane C.
Tavares, Davi Castro
Silciiano, Salvatore
Resumen
Carcasses of whales provide much valuable information on their natural history. However, some specimens cannot be identified
in the field due to the advanced state of decomposition. In this study, the DNA was extracted and the mitochondrial
cytochrome b gene was amplified by polymerase chain reaction and sequenced for four carcasses of possible mysticeti
(GEMM: 075, 088, 135 and GEMARS: 1302). A blast search using the nucleotide–nucleotide basic local alignment
(blastn) search tool was conducted using the generated sequences. Samples GEMM 075 and GEMARS 1302 showed 98% identity
to one sequence of Balaenoptera acutorostrata. Samples GEMM 088 and GEMM 135 showed 99% identity to sequences
from Balaenoptera edeni and Megaptera novaeangliae, respectively. A neighbour-joining tree was generated using sequences
from GenBank from all species of balaenopterid that occur on the coast of Brazil. The results showed that all carcasses analysed
were correspondent to species from the family Balaenopteridae already recorded in Brazil.