Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum

dc.creatorSantos, Catiule de Oliveira
dc.creatorCosta, Sidnei Ferro
dc.creatorSouza, Fabiana Santana
dc.creatorMendes, Jessica Mariane Ferreira
dc.creatorPinheiro, Cristiane Garboggini Melo de
dc.creatorMoreira, Diogo Rodrigo de Magalhães
dc.creatorSilva, Luciano Kalabric
dc.creatorLima, Valeria Marçal Felix de
dc.creatorOliveira, Geraldo Gileno de Sá
dc.date2021-03-08T12:26:01Z
dc.date2021-03-08T12:26:01Z
dc.date2021
dc.date.accessioned2023-09-26T20:10:25Z
dc.date.available2023-09-26T20:10:25Z
dc.identifierSANTOS, Catiule de Oliveira et al. Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum. Plos One, 2021.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/46290
dc.identifier10.1371/journal.pone.0239171
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8850404
dc.descriptionProgram for Technological Development of Tools for Health (PDTIS-FIOCRUZ) for the use of its facilities and to the Postgraduate Course of Biotechnology in Health and Investigative Medicine.
dc.descriptionrIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectLeishmaniose
dc.subjectCães
dc.subjectInterleucina-10
dc.subjectLeishmania infantum
dc.subjectTécnicas In Vitro
dc.subjectLeishmaniasis
dc.subjectDogs
dc.subjectInterleukin-10
dc.subjectLeishmania infantum
dc.subjectIn Vitro Techniques
dc.titleBlocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum
dc.typeArticle


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