Article
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum
Registro en:
SANTOS, Catiule de Oliveira et al. Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum. Plos One, 2021.
1932-6203
10.1371/journal.pone.0239171
Autor
Santos, Catiule de Oliveira
Costa, Sidnei Ferro
Souza, Fabiana Santana
Mendes, Jessica Mariane Ferreira
Pinheiro, Cristiane Garboggini Melo de
Moreira, Diogo Rodrigo de Magalhães
Silva, Luciano Kalabric
Lima, Valeria Marçal Felix de
Oliveira, Geraldo Gileno de Sá
Resumen
Program for Technological Development of Tools for Health
(PDTIS-FIOCRUZ) for the use of its facilities and to the Postgraduate Course of Biotechnology
in Health and Investigative Medicine. rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses,
thus preventing tissue damage. However, the restriction of inflammatory and immune
responses by IL-10 can also favor the development and/or persistence of chronic infections
or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum
develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune
responses to control the infection. These animals fail to mount specific lymphoproliferative
responses and produce interferon gamma and TNF-alpha that would activate macrophages
and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute
to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored
to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus
chromosome encoding this molecule, which was expressed in insect cells and
subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to
homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation
in dogs with leishmaniasis caused by L. infantum.