Article
Leishmania (Viannia) subgenus kDNA amplification for the diagnosis of mucosal leishmaniasis
Registro en:
DISCH, Jolande et al. Leishmania (Viannia) subgenus kDNA amplification for the diagnosis of mucosal leishmaniasis. Diagn. Microbiol. Infect. Dis., v. 51, n. 3, p. 185-190, Mar. 2005.
0732-8893
Autor
Disch, Jolande
Pedras, Mariana Junqueira
Orsini, Marcela
Pirmez, Claude
Oliveira, Maria Cláudia de
Castro, Marcelo
Rabello, Ana Lúcia Teles
Resumen
The utility of 2 polymerase chain reaction (PCR)–based assays amplifying genus or Viannia subgenus Leishmania minicircle kDNA for the diagnostics of ML was assessed. The Viannia subgenus product was yielded after PCR from isolates of L. (Viannia) braziliensis, L. (Viannia) colombiensis, and L. (Viannia) guyanensis, whereas no product was obtained with the non-Viannia–pertaining species: L. (Leishmania) amazonensis, L. (Leishmania) donovani, and L. (Leishmania) chagasi. With both assays, 11 of 13 (86.4%) patients with confirmed ML could be identified, whereas only 2 (16.7%) of these patients were positive by microscopy. All amplified genus-specific products gave a positive signal by hybridization with a Leishmania (Viannia) subgenus–specific radioactive probe. The Vianniasubgenus–specific kDNA PCR represents a sensitive and specific tool for the diagnosis of ML, remarkably improving the sensitivity of parasitological methods and offering an alternative for the radioactive-dependent assays for subgenus characterization