Article
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil
Registro en:
PEREIRA JÚNIOR, A. M. et al. Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil. Parasites & Vectors, v. 8, p.180, 2015.
1756-3305
10.1186/s13071-015-0789-2
Autor
Pereira Júnior, Antonio Marques
Teles, Carolina Bioni Garcia
Santos, Ana Paula de Azevedo dos
Rodrigues, Moreno de Souza
Marialva, Eric Fabrício
Pessoa, Felipe Arley Costa
Medeiros, Jansen Fernandes
Resumen
Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role
that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects
among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra
firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania
infection in those phlebotomine populations.
Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January,
February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were
extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania
species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing.
Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 – 58.26%)
and Nyssomyia antunesi (661 – 11.26%) were the most abundant species. Species richness was greater in terra
firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species)
were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus
davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of
Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality.Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate
that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment
of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric
stress during part of the year that could influence the local diversity, if compared with other studies. This is the first
report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA