Mitogenic response and cytokine production induced by cramoll 1,4 lectin in splenocytes of inoculated mice

dc.creatorMelo, C. M. L. de
dc.creatorMelo, H.
dc.creatorCorreia, M. T. S.
dc.creatorCoelho, L. C. B. B.
dc.creatorSilva, M. B. da
dc.creatorPereira, V. R. A.
dc.date2018-04-23T15:31:58Z
dc.date2018-04-23T15:31:58Z
dc.date2011
dc.date.accessioned2023-09-26T20:08:08Z
dc.date.available2023-09-26T20:08:08Z
dc.identifierDE MELO, C. M. L. et al. Mitogenic response and cytokine production induced by cramoll 1,4 lectin in splenocytes of inoculated mice. Scandinavian Journal of Immunology, v. 73, n. 2, p. 112–121, fev. 2011.
dc.identifier1365-3083
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26032
dc.identifier10.1111/j.1365-3083.2010.02490.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849374
dc.descriptionEste trabalho foi financiado pelo Conselho Nacional de Pesquisa (CNPq), pela Coordenação de Estudos Avançados em Educação Superior (CAPES) e pelo Centro de Pesquisas Aggeu Magalhães, da Fundação Oswaldo Cruz, em Recife (CPqAM / FIOCRUZ).
dc.descriptionCramoll 1,4 is a lectin with specific glucose/mannose binding, which is extracted from seeds of Cratylia mollis Mart. Many assays have shown the cytokine expression activity and anti-inflammatory profile of this lectin. The aim of this study was to evaluate the immunostimulatory response, in vitro, of splenocytes in mice previously inoculated, in vivo, with C. mollis (Cramoll 1,4) and Canavalia ensiformis (Con A) lectins. Results demonstrated higher proliferation indexes induced by Cramoll 1,4 than Con A lectin in relation to all experimental groups. Cramoll 1,4 and Con A also induced high levels of IL-2, IL-6, IFN-γ and nitric oxide production. Moreover, Cramoll 1,4 did not induce apoptosis and stimulated a significant number of cells in the S phase of the cell cycle. Results showed that Cramoll 1,4 lectin induces proliferative response and suggested that this lectin can be used as a mitogenic agent in immunostimulatory assays.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCramoll 1,4
dc.subjectCratylia mollis
dc.subjectresposta imunoestimuladora
dc.subjectLectinas
dc.subjectCramoll 1,4
dc.subjectCratylia mollis
dc.subjectimmunostimulatory response
dc.subjectlectin
dc.subjectAnimais
dc.subjectProliferação celular
dc.subjectCélulas Cultivadas
dc.subjectInterferon gama / biossíntese
dc.subjectInterferon gama / imunologia
dc.subjectInterleucina-2 / biossíntese
dc.subjectInterleucina-2 / imunologia
dc.subjectInterleucina-6 / biossíntese
dc.subjectInterleucina-6 / imunologia
dc.subjectLinfócitos / citologia
dc.subjectLinfócitos / imunologia
dc.subjectMasculino
dc.subjectRatos
dc.subjectMitose
dc.subjectÓxido Nítrico / biossíntese
dc.subjectLectinas vegetais / imunologia
dc.subjectBaço / citologia
dc.subjectBaço / imunologia
dc.titleMitogenic response and cytokine production induced by cramoll 1,4 lectin in splenocytes of inoculated mice
dc.typeArticle


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