Article
Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
Registro en:
OLIVEIRA, J. G. S. et al. Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis. Acta Tropica, v. 94, n. 1, p. 55-59, 2005.
0001-706X
10.1016/j.actatropica.2004.12.003
Autor
Oliveira, Jene Greyce Souza de
Novais, Fernanda Oliveira
Oliveira, Camila Indiani de
Cruz Junior, Antonio Clementino da
Campos, Léon Fábio
Rocha, Any V. da
Boaventura, Viviane Sampaio
Noronha, Almério Libório Lopes de
Costa, Jackson Mauricio Lopes
Barral, Aldina Maria Prado
Resumen
We evaluated the use of polymerase chain reaction (PCR) for diagnosis of mucosal leishmaniasis (ML) in an endemic area in
Acre, Brazil, where Leishmania braziliensis is present. Leishmania DNA was detected 34 of 35 cases, yielding a positivity rate
of 97.1%, which was higher than the positivity rates for all of the other diagnostic methods studied, namely Montenegro skin
test (MST), anti-Leishmania serological testing and microscopic examination of lesion biopsy specimens. These findings have
led us to propose guidelines for the diagnosis of ML that use PCR as the principal method of parasitological confirmation of
cases.