Otro
Bone healing in critical-size defects treated with platelet-rich plasma: a histologic and histometric study in rat calvaria
Registro en:
Journal of Periodontal Research. Oxford: Blackwell Publishing, v. 43, n. 2, p. 217-223, 2008.
0022-3484
10.1111/j.1600-0765.2007.01017.x
WOS:000253480700013
Autor
Messora, M. R.
Nagata, Maria José Hitomi
Mariano, R. C.
Dornelles, Rita Cassia Menegati
Bomfim, Suely Regina Mogami
Fucini, S. E.
Garcia, Valdir Gouveia
Bosco, Álvaro Francisco
Resumen
Background and objective: The purpose of this study was to analyze histologically the influence of autologous platelet-rich plasma on bone healing in surgically created critical-size defects in rat calvaria.Material adn Methods: Thirty-two rats were divided into two groups: the control group (group C) and the platelet-rich plasma group. An 8-mm-diameter critical-size defect was created in the calvarium of each animal. In group C the defect was filled by a blood clot only. In the platelet-rich plasma group, 0.35 mL of platelet-rich plasma was placed in the defect and covered by 0.35 mL of platelet-poor plasma. Both groups were divided into subgroups (n = 8) and killed at either 4 or 12 wk postoperatively. Histometric (using image-analysis software) and histologic analyses were performed. The amount of new bone formed was calculated as a percentage of the total area of the original defect. Percentage data were transformed into arccosine for statistical analysis (analysis of variance, Tukey, p < 0.05).Results: No defect completely regenerated with bone. The platelet-rich plasma group had a statistically greater amount of bone formation than group C at both 4 wk (17.68% vs. 7.20%, respectively) and 12 wk (24.69% vs. 11.65%, respectively) postoperatively.Conclusion: Within the limits of this study, it can be concluded that platelet-rich plasma placed in the defects and covered by platelet-poor plasma significantly enhanced bone healing in critical-size defects in rat calvaria.