Otro
Interaction between nitroheterocyclic compounds with beta-cyclodextrins: Phase solubility and HPLC studies
Registro en:
Journal of Pharmaceutical and Biomedical Analysis. Amsterdam: Elsevier B.V., v. 47, n. 4-5, p. 865-869, 2008.
0731-7085
10.1016/j.jpba.2008.04.022
WOS:000257220300032
0000-0002-2042-018X
Autor
de Melo, Nathalie F. S.
Grillo, Renato
Rosa, Andre Henrique
Fraceto, Leonardo Fernandes
Resumen
Chagas disease is a serious health problem for Latin America. Nitrofurazone (NF) and Hidroxymethylnitrofurazone (NFOH) are active against Trypanosoma cruzi. The effect of beta-cyclodextrin (beta-CD) and dimethyl-beta-cyclodextrin (DM-beta-CD) complexation on the UV absorption and retention time of nitrofurazone (NF) and its hydroxymethylated analog (NFOH) were studied in solution. The retention behavior was analyzed on a reversed phase C(18) column and the mobile phase used was acetonitrile-water (20/80 v/v), in which cyclodextrins (beta-CD or DM-beta-CD) were incorporated as a mobile phase additive. The decrease in the retention times of NF (or NFOH) with increasing concentration of HP-beta-CD enables the cleternnination of the complex stability constants by HPLC. A phase-solubility Study was performed. according to the method reported by Higuchi and Connors, to evaluate the changes of NF/NFOH in the complexation state, and the diagrams obtained suggested that it forms complexes with a stoichiometry of 1 : 1. This is an important Study for the characterization of potential formulations to be used as therapeutic options for the treatment of Chagas disease. (c) 2008 Elsevier B.V. All rights reserved.