Evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to pyrazinamide

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGhiraldi, L. D.
dc.creatorCampanerut, P. A. Z.
dc.creatorSpositto, F. L. E.
dc.creatorSato, D. N.
dc.creatorLeite, Clarice Queico Fujimura
dc.creatorHirata, M.
dc.creatorHirata, R. D. C.
dc.creatorCardoso, R. F.
dc.date2014-05-20T15:31:56Z
dc.date2016-10-25T18:07:58Z
dc.date2014-05-20T15:31:56Z
dc.date2016-10-25T18:07:58Z
dc.date2011-12-01
dc.date.accessioned2017-04-06T00:24:55Z
dc.date.available2017-04-06T00:24:55Z
dc.identifierClinical Microbiology and Infection. Malden: Wiley-blackwell, v. 17, n. 12, p. 1792-1797, 2011.
dc.identifier1198-743X
dc.identifierhttp://hdl.handle.net/11449/40951
dc.identifierhttp://acervodigital.unesp.br/handle/11449/40951
dc.identifier10.1111/j.1469-0691.2011.03508.x
dc.identifierWOS:000297012200012
dc.identifierhttp://dx.doi.org/10.1111/j.1469-0691.2011.03508.x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/883685
dc.descriptionThe microscopic observation drug susceptibility assay (MODS) was evaluated to determine susceptibility to pyrazinamide in Mycobacterium tuberculosis, and compared with the broth microdilution method (BMM), absolute concentration method (ACM), and pyrazinamidase (PZase) determination. We tested 34 M. tuberculosis clinical isolates (24 sensitive and eight resistant to pyrazinamide) and the control strains M. tuberculosis H37Rv (ATCC 27294) and Mycobacterium bovis AN5. The MODS, BMM, ACM and PZase determination provided results in average times of 6, 18, 28 and 7 days, respectively. All methods showed excellent sensitivity and specificity (p <0.05). of the methods studied, the MODS proved to be faster, efficient, inexpensive, and easy to perform. However, additional studies evaluating the MODS in differentiating pyrazinamide-resistant and pyrazinamide-susceptible M. tuberculosis must be conducted with a larger number of clinical isolates.
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationClinical Microbiology and Infection
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMicroscopic observation drug susceptibility assay (MODS)
dc.subjectpyrazinamide
dc.subjectresistance
dc.subjectsusceptibility testing
dc.subjecttuberculosis
dc.titleEvaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to pyrazinamide
dc.typeOtro


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