Canine cerebral leishmaniasis: Potential role of matrix metalloproteinase-2 in the development of neurological disease

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMelo, Guilherme D.
dc.creatorMarcondes, Mary
dc.creatorMachado, Gisele Fabrino
dc.date2014-05-20T15:31:22Z
dc.date2016-10-25T18:07:09Z
dc.date2014-05-20T15:31:22Z
dc.date2016-10-25T18:07:09Z
dc.date2012-08-15
dc.date.accessioned2017-04-06T00:21:47Z
dc.date.available2017-04-06T00:21:47Z
dc.identifierVeterinary Immunology and Immunopathology. Amsterdam: Elsevier B.V., v. 148, n. 3-4, p. 260-266, 2012.
dc.identifier0165-2427
dc.identifierhttp://hdl.handle.net/11449/40515
dc.identifierhttp://acervodigital.unesp.br/handle/11449/40515
dc.identifier10.1016/j.vetimm.2012.05.007
dc.identifierWOS:000309030400006
dc.identifierhttp://dx.doi.org/10.1016/j.vetimm.2012.05.007
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/883283
dc.descriptionMatrix metalloproteinases (MMPs) are a group of calcium- and zinc-dependent endopeptidases that are involved in maintaining the extracellular matrix. MMP-2 and MMP-9 are thought to be related to the disruption of the blood-brain-barrier (BBB) by their ability to cleave type IV collagen, the main component of the basal membrane. To establish the presence of MMP-2 and MMP-9 in the pathogenesis of canine cerebral leishmaniasis, we examined the levels of these metalloproteinases in the cerebrospinal fluid (CSF) and serum of dogs with visceral leishmaniasis and neurological symptoms (n = 16) and in the CSF and serum of uninfected healthy dogs (n =10) using zymography. In the CSF of dogs with cerebral leishmaniasis there was a massive presence of active MMP-2, whereas only the levels of both proMMP-2 and proMMP-9 were elevated in the serum. Although the detected MMP activity in the CSF might merely be related to CNS inflammation, these enzymes may also play a collaborative role in the disease progression. Both MMP-2 and MMP-9 are known to target critical constituents of the BBB, and once activated, they may promote cerebral barrier breakdown, allowing the entrance of inflammatory cells and proteins within the nervous system milieu. (c) 2012 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationVeterinary Immunology and Immunopathology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCentral nervous system
dc.subjectCerebrospinal fluid
dc.subjectInflammation
dc.subjectLeishmania chagasi
dc.subjectZymography
dc.titleCanine cerebral leishmaniasis: Potential role of matrix metalloproteinase-2 in the development of neurological disease
dc.typeOtro


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