Otro
Determination of cobalt marker in cow ruminal fluid by EDXRF and SRTXRF
Registro en:
X-ray Spectrometry. Hoboken: Wiley-blackwell, v. 40, n. 6, p. 424-426, 2011.
0049-8246
10.1002/xrs.1361
WOS:000297053400005
Autor
de Almeida, Eduardo
Massoni, Paulo Rogerio
Menegario, Amauri Antonio
Leite, Laudi Cunha
Duarte Lanna, Dante Pazzanese
do Nascimento Filho, Virgilio Franco
Resumen
This article describes a comparison of conventional energy-dispersive X-ray fluorescence (EDXRF) and synchrotron radiation total-reflection X-ray fluorescence (SRTXRF) for Co determination in ruminal fluid from Holstein cow. This element is used as marker for animal nutrition studies. For EDXRF, 200 mu l of the sample were dried on 6.35 mu m Mylar filmat 60 degrees C. The excitation was carried out using an X-ray tube with Mo target and Zr filter operated at 30 kV/ 20 mA. For SRTXRF, 10 mu l of the samplewere pipetted on a Lucite carrier and dried at 60 degrees C. In both the techniques, Ga was used as internal standard and the acquisition time was 200 s. The trueness of both techniques was evaluated through the standard addition method, the recoveries obtained by SRTXRF and EDXRF were 76 and 99%, and the limits of detection, 13 and 240 mu g l(-1), respectively. Copyright (C) 2011 John Wiley & Sons, Ltd.