Immunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumors

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorZuccari, D. A. P. C.
dc.creatorCastro, R.
dc.creatorJardim, B. V.
dc.creatorMancini, U. M.
dc.creatorPolachini, G. M.
dc.date2014-05-20T15:30:54Z
dc.date2016-10-25T18:06:35Z
dc.date2014-05-20T15:30:54Z
dc.date2016-10-25T18:06:35Z
dc.date2011-02-01
dc.date.accessioned2017-04-06T00:19:31Z
dc.date.available2017-04-06T00:19:31Z
dc.identifierArquivo Brasileiro de Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 63, n. 1, p. 28-35, 2011.
dc.identifier0102-0935
dc.identifierhttp://hdl.handle.net/11449/40187
dc.identifierhttp://acervodigital.unesp.br/handle/11449/40187
dc.identifierS0102-09352011000100005
dc.identifierWOS:000289643900005
dc.identifierS0102-09352011000100005.pdf
dc.identifierhttp://dx.doi.org/10.1590/S0102-09352011000100005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/882989
dc.descriptionForty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the gamma 2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 gamma 2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with gamma 2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 gamma 2 in malignant tumors. The results suggest that, in the future, low expression and/or degradation of laminin-332 gamma 2 chain in canine mammary tumors may be used as an indicator of malignant potential. However, further studies are necessary to corroborate these results.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherArquivo Brasileiro Medicina Veterinaria Zootecnia
dc.relationArquivo Brasileiro de Medicina Veterinária e Zootecnia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdog
dc.subjectadhesion molecule
dc.subjectcancer
dc.subjectwestern blot
dc.subjectRT-PCR
dc.titleImmunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumors
dc.typeOtro


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