Determination of malic acid in real samples by using enzyme immobilized reactors and amperometric detection

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorManzoli, A.
dc.creatorTomita, LSN
dc.creatorFertonani, F. L.
dc.creatorde Oliveira-Neto, G.
dc.creatorMascini, M.
dc.creatorYamanaka, H.
dc.date2014-05-20T15:25:46Z
dc.date2016-10-25T18:00:19Z
dc.date2014-05-20T15:25:46Z
dc.date2016-10-25T18:00:19Z
dc.date2004-07-01
dc.date.accessioned2017-04-05T23:54:00Z
dc.date.available2017-04-05T23:54:00Z
dc.identifierAnalytical Letters. Philadelphia: Taylor & Francis Inc., v. 37, n. 9, p. 1823-1832, 2004.
dc.identifier0003-2719
dc.identifierhttp://hdl.handle.net/11449/36117
dc.identifierhttp://acervodigital.unesp.br/handle/11449/36117
dc.identifier10.1081/AL-120039428
dc.identifierWOS:000223207900004
dc.identifierhttp://dx.doi.org/10.1081/AL-120039428
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/879700
dc.descriptionThe malate dehydrogenase (MDH) and ascorbate oxidase were immobilized independently, onto silanized controlled porous silica and packed in a tygon tube. The reactors were inserted in the flow system, and the malic acid was determined by measurement of NADH produced by enzymatic reaction. The NADH was reoxidized in a wall jet cell that consisted of spectrographic graphite, Ag/AgCl, KCl(sat), and steel needle as work, reference, and counter electrodes, respectively. The current intensities were measured at 390 mV. The malate calibration curve shows a linear range from 5.0 x 10(-6) to 1.0 x 10(-4) molL(-1), the lifetime was 40 analyses, after that a decrease of 20% on the response is observed. Three different citric juices were analyzed and a good correlation between the proposed method and spectrophotometric commercial kit were obtained.
dc.languageeng
dc.publisherTaylor & Francis Inc
dc.relationAnalytical Letters
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcitrus juice
dc.subjectmalic acid
dc.subjectmalate dehydrogenase
dc.subjectascorbic acid
dc.titleDetermination of malic acid in real samples by using enzyme immobilized reactors and amperometric detection
dc.typeOtro


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