Artigo
Lignases Production and Partial Purification of Mnp from Brazilian Fungal Isolate in Submerged Fermentation
Registro en:
Fermentation Technology, v. 1, n. 5, p. 1-7, 2012.
2167-7972
10.4172/2167-7972.1000106
Autor
Marti, Ferhan
Leao, Alcides Lopes [UNESP]
Melo, Itamar S de
Yan, Norman D.
Sain, Mohini
Resumen
The potential of ligninases as a green tool for effective valorization of lignin can be shown through enzymatic cocktails containing different lignin degrading enzymes. The present study deals with the screening of potential fungal strains useful for the liquefaction of bark containing lignin. Three different local isolates (Pleurotus ostreatus POS97/14, Pycnoporus sanguineus and the local isolated fungal strain) were selected out of ten different strains for ligninases production. Maximum production of enzymes was observed in the local isolated fungal strain after ten days in submerged fermentation. The isolated fungal strain produces ligninases mainly for manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related phenols. Furthermore, the partial purification for MnP was determined by FPLC and the molecular weight was evaluated by SDS-PAGE. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Departamento de Ciências Ambientais, Faculdade de Ciências Agronômicas de Botucatu, Botucatu, Rua Doutor José Barbosa de Barros, 1.780, Jardim Paraíso, CEP 18610307, SP, Brasil Center for Biocomposites and Biomaterials Processing, Faculty of Forestry, University of Toronto, Toronto, ON, Canada Department of Microbiology, Embrapa Environment, Jaguariúna, SP, Brazil Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Departamento de Ciências Ambientais, Faculdade de Ciências Agronômicas de Botucatu, Botucatu, Rua Doutor José Barbosa de Barros, 1.780, Jardim Paraíso, CEP 18610307, SP, Brasil