Hypospadias is a rare congenital condition characterized by abnormal development of the penile urethra, penis, scrotum and prepuce. The etiopathogenesis is still not fully elucidated, but it is based on a deficiency of testosterone during the critical stage of morphogenesis. At UNESP’s experimental farm was born a Mediterranean breed buffalo calf with congenital abnormalities in the reproductive system, being diagnosed with hypospadias.Universidade Estadual Paulista, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu