| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | Milagres, B. G. | |
| dc.creator | Neto, G. D. | |
| dc.creator | Kubota, L. T. | |
| dc.creator | Yamanaka, H. | |
| dc.date | 2014-05-20T15:23:56Z | |
| dc.date | 2016-10-25T17:57:59Z | |
| dc.date | 2014-05-20T15:23:56Z | |
| dc.date | 2016-10-25T17:57:59Z | |
| dc.date | 1997-07-30 | |
| dc.date.accessioned | 2017-04-05T23:44:32Z | |
| dc.date.available | 2017-04-05T23:44:32Z | |
| dc.identifier | Analytica Chimica Acta. Amsterdam: Elsevier B.V., v. 347, n. 1-2, p. 35-41, 1997. | |
| dc.identifier | 0003-2670 | |
| dc.identifier | http://hdl.handle.net/11449/34613 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/34613 | |
| dc.identifier | 10.1016/S0003-2670(97)00105-0 | |
| dc.identifier | WOS:A1997XQ51300006 | |
| dc.identifier | http://dx.doi.org/10.1016/S0003-2670(97)00105-0 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/878502 | |
| dc.description | An amperometric biosensor for salicylate detection was developed by immobilizing salicylate hydroxylase via glutaraldehyde onto a polypyrrole film doped with hexacyanoferrate, supported on a glassy carbon electrode surface. The sensor monitors the catechol produced in the enzymatic reaction on the film surface, at an applied potential of 150 mV vs. SCE. A [NADH]/[salicylate] ratio between 2 and 4 gave the best response. The biosensor presented the best performance in a solution with pH=7.4. The response time was about 40 s. A linear range of response was observed for salicylate concentrations between 1.0x10(-5) and 1.0x10(-4) mol l(-1) and the equation adjusted for this curve was I=(-0.04+/-0.01)+(11.4+/-0.2)[salicylate] with a correlation coefficient of 0.999 for n=6. The biosensor retains its activity for at least 10 days despite daily use. The results obtained using the biosensor for salicylate determination, in three different samples of antithermic drugs, presented a good correlation with the standard colorimetric method. | |
| dc.language | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation | Analytica Chimica Acta | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | salicylate biosensor | |
| dc.subject | polypyrrole film | |
| dc.subject | salicylate hydroxylase | |
| dc.subject | amperometry | |
| dc.title | A new amperometric biosensor for salicylate based on salicylate hydroxylase immobilized on polipyrrole film doped with hexacyanoferrate | |
| dc.type | Otro | |
