Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGomes, E.
dc.creatorSerzedello, A.
dc.date2014-05-20T15:23:54Z
dc.date2016-10-25T17:57:57Z
dc.date2014-05-20T15:23:54Z
dc.date2016-10-25T17:57:57Z
dc.date1996-01-01
dc.date.accessioned2017-04-05T23:44:23Z
dc.date.available2017-04-05T23:44:23Z
dc.identifierMicrobios. Cambridge: Faculty Press, v. 87, n. 353, p. 227-237, 1996.
dc.identifier0026-2633
dc.identifierhttp://hdl.handle.net/11449/34591
dc.identifierhttp://acervodigital.unesp.br/handle/11449/34591
dc.identifierWOS:A1996WJ24300003
dc.identifierhttp://cat.inist.fr/?aModele=afficheN&cpsidt=2559335
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/878484
dc.descriptionA ribonuclease was partially purified from the culture medium of Aspergillus flavipes (IZ:1501), after 96 h of cultivation by chromatography on DEAE-cellulose and Sephadex G100 columns. The molecular weight of the RNase was estimated to be 40 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 50-55 degrees C, respectively. Catalytic activity was inhibited by Zn+2, Fe+3, Hg+2 and Ag+ ions. The enzyme did not show an exact base specificity and produced four kinds of 3'-nucleotides from yeast RNA.
dc.languageeng
dc.publisherFaculty Press
dc.relationMicrobios
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectribonuclease
dc.subjectAspergillus
dc.subjectnucleotides
dc.titlePartial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
dc.typeOtro


Este ítem pertenece a la siguiente institución