Apoptosis and reduced microvascular density of the lamina propria during tooth eruption in rats

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorPizzol Júnior, José Paulo de
dc.creatorSasso-Cerri, Estela [UNESP]
dc.creatorCerri, Paulo Sérgio [UNESP]
dc.date2015-12-07T15:35:10Z
dc.date2015-12-07T15:35:10Z
dc.date2015
dc.date.accessioned2023-09-12T07:34:47Z
dc.date.available2023-09-12T07:34:47Z
dc.identifierhttp://dx.doi.org/10.1111/joa.12359
dc.identifierJournal Of Anatomy, v. 227, n. 4, p. 487-496, 2015.
dc.identifier1469-7580
dc.identifierhttp://hdl.handle.net/11449/131417
dc.identifier10.1111/joa.12359
dc.identifier4455630076841302
dc.identifier3278495911207882
dc.identifier26228092
dc.identifier0000-0001-5756-5828
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780698
dc.descriptionDuring tooth eruption, structural and functional changes must occur in the lamina propria to establish the eruptive pathway. In this study, we evaluate the structural changes that occur during lamina propria degradation and focus these efforts on apoptosis and microvascular density. Fragments of maxilla containing the first molars from 9-, 11-, 13- and 16-day-old rats were fixed, decalcified and embedded in paraffin. The immunohistochemical detection of vascular endothelial growth factor (VEGF), caspase-3 and MAC387 (macrophage marker), and the TUNEL method were applied to the histological molar sections. The numerical density of TUNEL-positive cells and VEGF-positive blood vessel profiles were also obtained. Data were statistically evaluated using a one-way anova with the post-hoc Kruskal-Wallis or Tukey test and a significance level of P ≤ 0.05. Fragments of maxilla were embedded in Araldite for analysis under transmission electron microscopy (TEM). TUNEL-positive structures, fibroblasts with strongly basophilic nuclei and macrophages were observed in the lamina propria at all ages. Using TEM, we identified processes of fibroblasts or macrophages surrounding partially apoptotic cells. We found a high number of apoptotic cells in 11-, 13- and 16-day-old rats. We observed VEGF-positive blood vessel profiles at all ages, but a significant decrease in the numerical density was found in 13- and 16-day-old rats compared with 9-day-old rats. Therefore, the establishment of the eruptive pathway during the mucosal penetration stage depends on cell death by apoptosis, the phagocytic activity of fibroblasts and macrophages, and a decrease in the microvasculature due to vascular cell death. These data point to the importance of vascular rearrangement and vascular neoformation during tooth eruption and the development of oral mucosa.
dc.descriptionDepartment of Morphology and Genetics, Federal University of São Paulo (UNIFESP), São Paulo, SP, Brazil.
dc.descriptionDental School - Laboratory of Histology and Embryology, UNESP - São Paulo State University, Araraquara, SP, Brazil.
dc.descriptionDental School - Laboratory of Histology and Embryology, UNESP - São Paulo State University, Araraquara, SP, Brazil.
dc.format487-496
dc.languageeng
dc.publisherAnatomical Society.
dc.relationJournal Of Anatomy
dc.relation1,078
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectApoptosis
dc.subjectEruptive pathway
dc.subjectLamina propria
dc.subjectMicrovasculature
dc.subjectTooth eruption
dc.titleApoptosis and reduced microvascular density of the lamina propria during tooth eruption in rats
dc.typeArtigo


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