Artigo
A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine
Registro en:
Experientia, v. 49, n. 9, p. 810-813, 1993.
0014-4754
10.1007/BF01923555
WOS:A1993LY25500015
2-s2.0-0027366658
0804793944846367
Autor
Foresti, F. [UNESP]
Oliveira, C. [UNESP]
Foresti de Almeida-Toledo, L. [UNESP]
Resumen
This paper describes a new technique for preparing mitotic fish chromosomes using short-term in vitro treatment with colchicine. The results show that a large number of good quality metaphases (many suitable for chromosome banding) can be obtained by this technique, which requires an average of 1 h and 30 min for all steps. The procedure considerably reduces the time normally required for chromosome preparations in fish. Departamento de Morfologia Instituto de Biociencias UNESP, 18618-000 Botucatu USP,INST BIOCIENCIAS,DEPT BIOL,SAO PAULO,BRAZIL Departamento de Morfologia Instituto de Biociencias UNESP, 18618-000 Botucatu