Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniv Valencia
dc.creatorDavolos, Camila C. [UNESP]
dc.creatorHernandez-Martinez, Patricia
dc.creatorCrialesi-Legori, Paula C. B. [UNESP]
dc.creatorDesiderio, Janete A. [UNESP]
dc.creatorFerre, Juan
dc.creatorEscriche, Baltasar
dc.creatorLemos, Manoel Victor F. [UNESP]
dc.date2015-10-21T21:12:44Z
dc.date2015-10-21T21:12:44Z
dc.date2015-05-01
dc.date.accessioned2023-09-12T06:55:45Z
dc.date.available2023-09-12T06:55:45Z
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0022201115000385
dc.identifierJournal Of Invertebrate Pathology. San Diego: Academic Press Inc Elsevier Science, v. 127, p. 32-34, 2015.
dc.identifier0022-2011
dc.identifierhttp://hdl.handle.net/11449/129492
dc.identifier10.1016/j.jip.2015.01.013
dc.identifierWOS:000354503500005
dc.identifier1295051340591836
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8778831
dc.descriptionSugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac,Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managing this pest. (C) 2015 Elsevier Inc. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionSpanish Ministry of Science and Innovation
dc.descriptionGeneralitat Valenciana
dc.descriptionEuropean FEDER
dc.descriptionUniv Estadual Paulista Julio de Mesquita Filho UN, Dept Appl Biol, BR-14884900 Jaboticabal, SP, Brazil
dc.descriptionUniv Valencia, Fac CC Biol, Dept Genet, E-46100 Valencia, Spain
dc.descriptionUniv Estadual Paulista Julio de Mesquita Filho UN, Dept Appl Biol, BR-14884900 Jaboticabal, SP, Brazil
dc.descriptionFAPESP: 2009/18544-1
dc.descriptionCAPES: CAPES/DGU 194/09
dc.descriptionCAPES: BEX-2336/11-1
dc.descriptionSpanish Ministry of Science and Innovation: AGL2012-39946-C02-01
dc.descriptionSpanish Ministry of Science and Innovation: PHB2008-0113-PC
dc.descriptionGeneralitat Valenciana: PROMETEO 2011/044
dc.format32-34
dc.languageeng
dc.publisherElsevier B.V.
dc.relationJournal Of Invertebrate Pathology
dc.relation2.511
dc.relation0,929
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectInsect resistance management
dc.subjectInsect corn pest
dc.subjectInsecticidal proteins
dc.subjectInsect control
dc.subjectBinding site models
dc.titleBinding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)
dc.typeArtigo


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