Artigo
Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)
Registro en:
Journal Of Invertebrate Pathology. San Diego: Academic Press Inc Elsevier Science, v. 127, p. 32-34, 2015.
0022-2011
10.1016/j.jip.2015.01.013
WOS:000354503500005
1295051340591836
Autor
Davolos, Camila C. [UNESP]
Hernandez-Martinez, Patricia
Crialesi-Legori, Paula C. B. [UNESP]
Desiderio, Janete A. [UNESP]
Ferre, Juan
Escriche, Baltasar
Lemos, Manoel Victor F. [UNESP]
Resumen
Sugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac,Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managing this pest. (C) 2015 Elsevier Inc. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Spanish Ministry of Science and Innovation Generalitat Valenciana European FEDER Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Appl Biol, BR-14884900 Jaboticabal, SP, Brazil Univ Valencia, Fac CC Biol, Dept Genet, E-46100 Valencia, Spain Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Appl Biol, BR-14884900 Jaboticabal, SP, Brazil FAPESP: 2009/18544-1 CAPES: CAPES/DGU 194/09 CAPES: BEX-2336/11-1 Spanish Ministry of Science and Innovation: AGL2012-39946-C02-01 Spanish Ministry of Science and Innovation: PHB2008-0113-PC Generalitat Valenciana: PROMETEO 2011/044