The aim of this study was to evaluate the potential of the successive projection algorithm for interval selection in partial least squares (iSPA-PLS) together with near-infrared reflectance spectroscopy (NIRS) as a feasible method to determine the total anthocyanin content (TAC) of intact jaboticaba fruit [Myrciaria jaboticaba (Vell.) O. Berg]. A total of 579 jaboticaba fruit were collected in three different harvests in three separate years (2011 and 2013). The correlation coefficients between the predicted and measured TAC were between 0.65 and 0.89, the RMSEPs were 7.55 g kg(-1) and 9.35 g kg(-1) (good accuracy) for prediction set, respectively. The RPD ratios for TAC were in the range of 2.57-3.19 with iSPA-PLS, which showed better predictive performance (acceptable precision). These results suggest that the NIR spectroscopy and wavelength selection (iSPA-PLS) algorithm can be used to determine the TAC of intact jaboticaba fruit. (C) 2014 Elsevier Ltd. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPERNPró-Reitoria de Pesquisa da Universidade de São PauloFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Av. do Café, s/n. Campus Universitário da USP, Ribeirão Preto, 14040-903 São Paulo, BrazilUFRN, Instituto de Química, PPGQ, Química Biológica e Quimiometria, 59072-970 Natal, RN, BrazilUSP, Faculdade de Medicina de Ribeirão Preto, Av. do Café, s/n, Campus Universitário da USP, Ribeirão Preto, 14040-903 São Paulo, Brazil.UNESP, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Produção Vegetal, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, 14884-900 São Paulo, BrazilCNPq: 070/2012FAPERN: 005/2012Pro-Reitoria de Pesquisa da Universidade de São Paulo: 10.1.25403.1.1Pró-Reitoria de Pesquisa da Universidade de Sao Paulo: 2011.1.6858.1.8FAPESP: 2008/51408-1CNPq: 477386/2011-3