Artigo
The cosmetic dye quinoline yellow causes DNA damage in vitro
Registro en:
Mutation Research-genetic Toxicology And Environmental Mutagenesis, v. 777, p. 54-61, 2015.
1383-5718
10.1016/j.mrgentox.2014.11.003
WOS:000351651200007
0000-0002-2296-1393
Autor
Drumond Chequer, Farah Maria
Venancio, Vinicius de Paula
Souza Prado, Maira Rocha de
Silva e Cunha Junior, Luiz Raimundo Campos da
Lizier, Thiago Mescoloto [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
Burbano, Rommel Rodriguez
Pires Bianchi, Maria Lourdes
Greggi Antunes, Lusania Maria
Resumen
Quinoline yellow (QY) is a chinophthalon derivative used in cosmetic compositions for application to the skin, lips, and/or body surface. However, regulatory data about the genotoxicity and/or mutagenicity of this compound are still controversial. Therefore, this work evaluated the genotoxicity of QY using the comet assay and the cytokinesis-block micronucleus cytome assay (CBMN-Cyt) in the metabolically competent cell line HepG2, which closely mimics phase I metabolism. This research also identified the products formed after electrochemical oxidation of the QY dye, which simulates hepatic biotransformation. The primary products generated after the oxidation process were analyzed by High Performance Liquid Chromatography coupled with a Diode Array Detector (HPLC/DAD), which detected the production of 4,4'-diaminodiphenylmethane, 2-methoxy-5-methylaniline and 4,4'-oxydianiline. The results demonstrated that low (from 0.5 to 20 mu g mL(-1)) QY concentrations were genotoxic in HepG2 cells on both assays and those harmful compounds were detected after the oxidation process. Our findings suggest that this colorant could cause harmful effects to humans if it is metabolized or absorbed through the skin. (C) 2014 Elsevier B.V. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol &Bromatol, BR-14040903 Ribeirao Preto, SP, Brazil Univ Fed Minas Gerais, Fac Fed Minas Gerais, Dept Anal Clin &Toxicol, BR-31270901 Belo Horizonte, MG, Brazil Fed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66059 Belem, Para, Brazil Univ Estadual Paulista, UNESP, Dept Quim Analit, Inst Quim, BR-14800900 Araraquara, SP, Brazil Univ Estadual Paulista, UNESP, Dept Quim Analit, Inst Quim, BR-14800900 Araraquara, SP, Brazil FAPESP: 2011/01755-0 FAPESP: 2011/14115-9