Artigo
A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Registro en:
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.
1517-8382
10.1590/S1517-838246120130851
S1517-83822015000100113
WOS:000355769900013
S1517-83822015000100113.pdf
1425748916849376
0000-0001-5627-8616
Autor
Angelini, Leticia Mara Lima [UNESP]
Silva, Amanda Ribeiro Martins da [UNESP]
Rocco, Lucas de Freitas Coli [UNESP]
Milagre, Cintia Duarte de Freitas [UNESP]
Resumen
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Universidade Estadual Paulista, Departamento de Biologia, Instituto de Biociências de Rio Claro Universidade Estadual Paulista, Departamento de Química Orgânica, Instituto de Química de Araraquara