Artigo
Androgen therapy reverses injuries caused by ethanol consumption in the prostate: testosterone as a possible target to ethanol-related disorders
Registro en:
Life Sciences, v. 120, p. 22-30, 2015.
0024-3205
10.1016/j.lfs.2014.11.003
WOS:000348693700004
Autor
Mendes, Leonardo O.
Scarano, Wellerson R. [UNESP]
Rochel-Maia, Sabrina S.
Fioruci-Fontaneli, Beatriz A.
Chuffa, Luiz G. A. [UNESP]
Anselmo-Franci, Janete A.
Martinez, Francisco E. [UNESP]
Resumen
Aims: Chronic ethanol consumption leads to reproductive damages, since it can act directly in the tissues or indirectly, causing a hormonal imbalance. Prostate is a hormone-dependent gland and, consequently, susceptible to ethanol. The potential of testosterone therapy in the ethanol-related disorders was investigated in the prostate microenvironmentMain methods: UChB rats aged 90 days were divided into 2 experimental groups (n = 20): C: drinking water only and EtOH: drinking 10% (v/v) ethanol at >2 g/kg body weight! day + water. At 150 days old, 10 rats from each group received subcutaneous injections of testosterone cypionate (5 mg/kg body weight) diluted in corn oil every other day for 4 weeks, constituting T and EtOH+T, while the remaining animals received corn oil as vehicle. Animals were euthanized at 180 days old, by decapitation. Blood was collected to obtain hormone concentrations and ventral prostate was dissected and processed for light microscope and molecular analyses.Key findings: Ventral prostate weight, plasma testosterone and DHT and intraprostatic testosterone concentrations were increased after testosterone treatment Plasma estradiol level was reduced in the EtOH +T. Inflammatory foci, metaplasia and epithelial atrophy were constantly found in the prostate of EtOH and were not observed after hormonal therapy. No differences were found in the expression of AR, ER beta and DACH-1. Additionally, testosterone treatment down-regulated ER alpha and increased the e-cadherin and a-actinin immunoreactivities.Significance: Testosterone was able to reverse damages caused by ethanol consumption in the prostate microenvironment and becomes a possible target to be investigated to ethanol-related disorders. (C) 2014 Elsevier Inc. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) State Univ Campinas UNICAMP, Struct &Cell Biol Program, Campinas, SP, Brazil Univ Estadual Paulista, UNESP, Inst Biosci, Dept Morphol, BR-18618910 Botucatu, SP, Brazil Inst Biosci Humanities &Exact Sci, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil Univ Estadual Paulista, UNESP, Inst Biosci, Dept Anat, BR-18618910 Botucatu, SP, Brazil Univ Sao Paulo, USP, Dept Morphol Stomatol &Physiol, BR-14049 Ribeirao Preto, SP, Brazil Univ Estadual Paulista, UNESP, Inst Biosci, Dept Morphol, BR-18618910 Botucatu, SP, Brazil Univ Estadual Paulista, UNESP, Inst Biosci, Dept Anat, BR-18618910 Botucatu, SP, Brazil FAPESP: 2009/54606-1 FAPESP: 2011/05936-9 FAPESP: 2012/00917-9