Artigo
Lycopene-rich tomato oleoresin modulates plasma adiponectin concentration and mRNA levels of adiponectin, SIRT1, and FoxO1 in adipose tissue of obese rats
Registro en:
Human &experimental Toxicology. London: Sage Publications Ltd, v. 34, n. 6, p. 612-619, 2015.
0960-3271
10.1177/0960327114551395
WOS:000355334400005
2940051650846541
Autor
Luvizotto, R. A. M. [UNESP]
Nascimento, A. F.
Miranda, N. C. M. [UNESP]
Wang, X-D
Ferreira, A. L. A. [UNESP]
Resumen
Aim: To investigate whether lycopene can modulate adiponectin levels and SIRT1 and FoxO1 gene expression in the adipose tissue of diet-induced obese rats.Methods: Male Wistar rats were first fed with hypercaloric diet (HD, n = 12) for 6 weeks, and afterward, these rats were randomly assigned to receive HD (n = 6) or HD with lycopene-rich tomato oleoresin (equivalent to lycopene 10 mg/kg body weight (BW)/day, HD + L, n = 6) by gavage for additional 6 weeks. Plasma lycopene and adiponectin levels were analyzed by high-performance liquid chromatography and immunoassay, respectively. The messenger RNA (mRNA) expressions of adiponectin, Sirtuin 1 (SIRT1), Forkhead box O 1 (FoxO1), fatty acid translocase/cluster of differentiation 36 (FAT/CD36), and PPAR in adipose tissues were determined by quantitative polymerase chain reaction.Results: Lycopene was detected in the plasma of rats in HD + L group but not in the HD group. Although both BW and adiposity were not different between the two groups, there was a significant increase in both plasma concentration and mRNA expression of adiponectin in the adipose tissue of the HD + L group. In addition, the lycopene supplementation upregulated mRNA expressions of SIRT1, FoxO1, and FAT/CD36 but downregulated PPAR in adipose tissue of obese rats.Conclusion: These data suggest that lycopene, in the concentration used, is not toxic and also its health benefits in adipose tissue may play a role against obesity-related complications. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) U.S. Department of Agriculture Institute of Health Science, Federal University of Mato Grosso (UFMT), Sinop, Mato Grosso, Brazil Department of Internal Medicine, Botucatu Medical School (UNESP), Botucatu, São Paulo, Brazil Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts, USA Department of Internal Medicine, Botucatu Medical School (UNESP) FAPESP: 2010/06100-9 FAPESP: 2010/19746-4 FAPESP: 2011/19934-8 FAPESP: 2011/22786-0 U.S. Department of Agriculture: 1950-51000-056