dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorDasilva, R.
dc.creatorYim, D. K.
dc.creatorAsquieri, E. R.
dc.creatorPark, Y. K.
dc.date2014-05-20T15:22:30Z
dc.date2016-10-25T17:56:11Z
dc.date2014-05-20T15:22:30Z
dc.date2016-10-25T17:56:11Z
dc.date1993-10-01
dc.date.accessioned2017-04-05T23:36:56Z
dc.date.available2017-04-05T23:36:56Z
dc.identifierRevista de Microbiologia. São Paulo: Soc Brasileira Microbiologia, v. 24, n. 4, p. 269-274, 1993.
dc.identifier0001-3714
dc.identifierhttp://hdl.handle.net/11449/33455
dc.identifierhttp://acervodigital.unesp.br/handle/11449/33455
dc.identifierWOS:A1993ND09500011
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_issues&pid=0001-3714&lng=en&nrm=iso
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/877571
dc.descriptionIn order to obtain cellulases that improve the detergency of laundry detergent products, two alkalophilic microorganims, Bacillus sp B38-2 and Streptomyces sp S36-2, were isolated from soil and compost by incubating samples in enrichment culture medium containing CMC and Na2CO3 at pH9.6. It was found that they secrete a constitutive extracellular alkaline carboxymethyl cellulase (CMCase) in high quantity. The maximum enzyme activity was observed between 48hr to 72 hr at 30-degrees-C for the Streptomyces and between 72hr to 96hr at 35-degrees-C for the Bacillus. The optimum pH and temperature of the crude enzyme activities ranged from 6.0 to 7.0 at 55-degrees-C for the Streptomyces and 7.0 to 8.0 at 60-degrees-C for the Bacillus. Two crude CMCases activities were termostable at 45-degrees-C for 1hr and the both crude enzyme activities of the Bacillus as of the Streptomyces were stable at pH 5.0 to 9.0 after pH treatments in various buffer solutions at 30-degrees-C for 24hr.
dc.languageeng
dc.publisherSoc Brasileira Microbiologia
dc.relationRevista de Microbiologia
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectALKALINE CELLULASES
dc.subjectALKALOPHILIC MICROORGANISMS
dc.subjectLAUNDRY DETERGENT
dc.subjectALKALOPHILIC BACILLUS
dc.subjectALKALOPHILIC STREPTOMYCES
dc.titlePRODUCTION OF MICROBIAL ALKALINE CELLULASE AND STUDIES OF THEIR CHARACTERISTICS
dc.typeOtro


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