Artigo
Production of β-(1,3)-glucanases by Trichoderma harzianum Rifai: Optimization and Application to Produce Gluco-oligosaccharides from Paramylon and Pustulan
Registro en:
Fermentation Technology, v. 1, p. 1-5, 2011.
2167-7972
10.4172/2167-7972.1000102
ISSN2167-7972-2011-01-01-15.pdf
9424175688206545
4840067353836807
Autor
Giese, E. C [UNESP]
Dekker, Robert F. H.
Barbosa, Aneli de Melo [UNESP]
Silva, Maria de lourdes Corradi da [UNESP]
Silva, Roberto da [UNESP]
Resumen
β-(1→3)-Glucanases were produced by Trichoderma harzianum Rifai PAMB-86 cultivated on botryosphaeran in a bench-fermenter and optimised by the response surface method. Maximal enzyme titres occurred at 5 days, initial pH 5.5 and aeration of 1.5vvm. β-(1→3)-The β-glucanolytic enzyme complex produced by T. harzianum Rifai PAMB- 86 was fractionated by gel filtration into 2 fractions (F-I, F-II), and employed to produce gluco-oligosaccharides from algal paramylon ((1→3)-β-D-glucan) and lichen pustulan ((1→6)-β-D-glucan). Both enzymes attacked paramylon to the extent of ~15-20% in 30 min releasing glucose and laminaribiose as major end-products, and laminarioligosaccharides of degree of polymerization (DP) ≥3. Only F-I degraded pustulan resulting in ~2% degradation at 30 min, with glucose, gentiobiose and gentio-oligosaccharides of DP ≥4 as major products. The difference in the nature of the hydrolysis products can be explained by the substrate specificities of each enzyme fraction, and the structural differences of the β-D-glucans attacked. Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, Rua Cristovão Colombo, 2265, Jardim Nazareth, CEP 15054-000, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, Rua Cristovão Colombo, 2265, Jardim Nazareth, CEP 15054-000, SP, Brasil Biorefining Research Institute, Lakehead University, Thunder Bay, ON, Canada P7B 5E1 Departamento de Bioquímica e Biotecnologia, CCE, Universidade Estadual de Londrina, UEL, Londrina, Paraná, Brazil Departamento de Física, Química e Biologia, Universidade Estadual Paulista, FCT, Presidente Prudente, São Paulo, Brazil