Artigo
Age- and gender-related changes in glucose homeostasis in glucocorticoid-treated rats
Registro en:
Canadian Journal Of Physiology And Pharmacology. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 92, n. 10, p. 867-878, 2014.
0008-4212
10.1139/cjpp-2014-0259
WOS:000343099500009
1445259468526188
0000-0002-0970-4288
Autor
Santos, Cristiane dos
Ferreira, Francielle Batista D.
Goncalves-Neto, Luiz M.
Taboga, Sebastiao Roberto [UNESP]
Boschero, Antonio Carlos
Rafacho, Alex
Resumen
The disruption to glucose homeostasis upon glucocorticoid (GC) treatment in adult male rats has not been fully characterized in older rats or in females. Thus, we evaluated the age-and gender-related changes in glucose homeostasis in GC-treated rats. We injected male and female rats at 3 months and 12 months of age with either dexamethasone (1.0 mg/kg body mass, intraperitoneally) or saline, daily for 5 days. All of the GC-treated rats had decreased body mass and food intake, and adrenal hypotrophy. Increased glycemia was observed in all of the GC-treated groups and only the 3-month-old female rats were not glucose intolerant. Dexamethasone treatment resulted in hyperinsulinemia and hypertriacylglyceridemia in all of the GC-treated rats. The glucose-stimulated insulin secretion (GSIS) was higher in all of the dexamethasone-treated animals, but it was less pronounced in the older animals. The beta-cell mass was increased in the younger male rats treated with dexamethasone. We conclude that dexamethasone treatment induces glucose intolerance in both the 3- and 12-month-old male rats as well as hyperinsulinemia and augmented GSIS. Three-month-old female rats are protected from glucose intolerance caused by GC, whereas 12-month-old female rats developed the same complications that were present in 3- and 12-month-old male rats. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Instituto Nacional de Ciencia e Tecnologia: Obesidade e Diabetes Fed Univ Santa Catarina UFSC, Dept Physiol Sci, Ctr Biol Sci, BR-88040900 Florianopolis, SC, Brazil Sao Paulo State Univ UNESP, Dept Biol, Inst Biosci Humanities & Exact Sci, Sao Jose Do Rio Preto, Brazil State Univ Campinas UNICAMP, Dept Struct & Funct Biol, Inst Biol, Campinas, SP, Brazil State Univ Campinas UNICAMP, OCRC, Campinas, SP, Brazil Sao Paulo State Univ UNESP, Dept Biol, Inst Biosci Humanities & Exact Sci, Sao Jose Do Rio Preto, Brazil CNPq: 471397/2011-3