Artigo
Antibiofilm activity of irrigating solutions associated with cetrimide. Confocal laser scanning microscopy
Registro en:
International Endodontic Journal. Hoboken: Wiley-blackwell, v. 47, n. 11, p. 1058-1063, 2014.
0143-2885
10.1111/iej.12248
WOS:000343760600006
Autor
Guerreiro-Tanomaru, J. M. [UNESP]
Nascimento, C. A. [UNESP]
Faria-Junior, N. B. [UNESP]
Graeff, M. S. Z.
Watanabe, E.
Tanomaru-Filho, M. [UNESP]
Resumen
AimTo evaluate the antibiofilm activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) solutions associated with cetrimide (CTR), and QMiX using confocal laser scanning microscopy.MethodologyEnterococcus faecalis (ATCC- 29212) biofilms were induced on bovine dentine blocks for 14days. The dentine blocks containing biofilm were immersed for 1min in the following solutions: 2.5% NaOCl; 2.5% NaOCl+0.2% CTR; 2% CHX; 2% CHX+0.2% CTR; 0.2% CTR; QMiX. After contact with the solutions, the dentine blocks were stained with Live/Dead((R)) BacLight for analysis of the remaining biofilm using confocal laser scanning microscope. Images were evaluated using the BioImage_L software to determine the total biovolume (m(3)), the green biovolume (live cells) (m(3)) and the percentage of substrate coverage (%). The data were subjected to nonparametric statistical test using Kruskal-Wallis and Dunn's tests at 5% significance level.ResultsAfter exposure to irrigants, the total biovolume observed for CHX, CHX+CTR, CTR, QMiX was similar to distilled water (P>0.05). NaOCl and NaOCl+CTR had the lowest total and green biovolume. The CTR and QMiX had intermediate green biovolume, with greater antibacterial activity than CHX and CHX+CTR (P<0.05). The NaOCl and NaOCl+CTR solutions were associated with microorganism removal and substrate cleaning ability.ConclusionsNaOCl and NaOCl+CTR solutions were effective on microorganism viability and were able to eliminate biofilm. The addition of cetrimide did not influence antibacterial activity. UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil Univ Sao Paulo, Bauru Dent Sch, Integrated Res Ctr, Bauru, SP, Brazil Univ Sao Paulo, Ribeirao Preto Dent Sch, Dept Restorat Dent, Ribeirao Preto, SP, Brazil UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil