Artigo
Similarity of heterochromatic regions in the stingless bees (Hymenoptera: Meliponini) revealed by chromosome painting
Registro en:
Caryologia. Florence: Univ Florence Botany Inst, v. 67, n. 3, p. 222-226, 2014.
0008-7114
10.1080/0144235X.2014.974349
WOS:000345995400004
0804793944846367
Autor
Lopes, Denilce Meneses
Fernandes, Anderson
Diniz, Debora
Sobrinho Scudeler, Patricia Elda [UNESP]
Foresti, Fausto [UNESP]
Oliveira Campos, Lucio Antonio de
Resumen
Most Meliponini share a distinctive pattern of heterochromatin distribution in relation to other bees. In general, they present one euchromatic and one heterochromatic chromosome arm, a feature explained by minimum interaction theory, which involved centric fissions followed by heterochromatin addition. In this work, two Meliponini with a distinct pattern of heterochromatin distribution, Tetragonisca fiebrigi and Melipona rufiventris, were analyzed using chromosomal microdissection of the heterochromatin region followed by FISH (fluorescent in situ hybridization). Hybridization revealed FISH signals equivalent to location of the isolated fragment that were widespread over heterochromatic portions of other chromosomes. This result showed that the heterochromatic sequences were very similar among chromosomes in the same species. Cross-hybridization of each probe in M. rufiventris and T. fiebrigi yielded no signals, revealing that both species presented differentiated and non-homologous heterochromatin sequences. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Mato Grosso (Fapemat) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Univ Fed Vicosa, Dept Biol Geral, Vicosa, MG, Brazil Univ Estado Mato Grosso, Dept Ciencias Biol, Tangara Da Serra, MT, Brazil Univ Estadual Sudoeste Bahia, Dept Ciencias Biol, Jequie, BA, Brazil UNESP, Dept Morfol, Botucatu, SP, Brazil UNESP, Dept Morfol, Botucatu, SP, Brazil