| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | Oliveira Amorim, Jose Benedito | |
| dc.creator | Musa-Aziz, Raif | |
| dc.creator | Lessa, Lucilia M. A. | |
| dc.creator | Malnic, Gerhard | |
| dc.creator | Fonteles, Manasses Claudino | |
| dc.date | 2014-05-20T15:21:05Z | |
| dc.date | 2016-10-25T17:54:22Z | |
| dc.date | 2014-05-20T15:21:05Z | |
| dc.date | 2016-10-25T17:54:22Z | |
| dc.date | 2006-10-01 | |
| dc.date.accessioned | 2017-04-05T23:29:19Z | |
| dc.date.available | 2017-04-05T23:29:19Z | |
| dc.identifier | Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 84, n. 10, p. 1003-1010, 2006. | |
| dc.identifier | 0008-4212 | |
| dc.identifier | http://hdl.handle.net/11449/32267 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/32267 | |
| dc.identifier | 10.1139/Y06-044 | |
| dc.identifier | WOS:000243731100006 | |
| dc.identifier | http://dx.doi.org/10.1139/Y06-044 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/876640 | |
| dc.description | The effect of uroguanylin (UGN) oil K(+) and H(+) secretion in the renal tubules of the rat kidney was studied using in vivo stationary microperfusion. For the study of K(+) secretion, a tubule was Punctured to inject a column of FDC-green-colored Ringer's solution with 0.5 mmol KCI/L 10(-6)(mol UGN/L, and oil was Used to block fluid flow. K(+) activity and transepithelial potential differences (PD) were measured with double microelectrodes (K(+) ion-selective resin vs. reference) in the distal tubules of the same nephron. During perfusion, K(+) activity rose exponentially, from 0.5 mmol/L to stationary concentration, allowing for the calculation of K(+) secretion J(K)). JK increased from 0.63 +/- 0.06 nmol.cm(-2).s(-1) in the control croup to 0.85 +/- 0.06 in the UGN group (p < 0.01). PD was -51.0 +/- 5.3 mV in the control group and -50.3 +/- 4.98 mV in the UGN group. In the presence of 10(-7) mol iberiotoxin/L, the UGN effect was abolished: JK was 0.37 +/- 0.038 nmol-cm(-2).s(-1) in the absence of, and 0.38 +/- 0.025 in the presence of, UGN. indicating its action oil rnaxi-K channels. In another series of experiments, renal tubule acidification was studied, using similar method: proximal and distal tubules were perfused with solutions containing 25 mmol NaHCO(3)/L. Acidification half-time was increased both in proximal and distal segments and, as a consequence, bicarbonate reabsorption decreased in the presence of UGN (in proximal tubules, from 2.40 +/- 0.26 to 1.56 +/- 0.21 nmol-cm(-2).s(-1)). When the Na(+)/H(+) exchanger was inhibited by 10(-4) mol hexamethylene amiloride (HMA)/L, the control and UGN groups were not significantly different. In the late distal tubule, after HMA, UGN significantly reduced J(HCO3)(-). indicating all effect of UGN oil H(+)-ATPase. These data show that UGN stimulated J(K)(+) by actin, oil maxi-K channels. and decreased J(HCO3)(-) by acting on NHE3 in proximal and H(+)-ATPase in distal tubules. | |
| dc.language | eng | |
| dc.publisher | Natl Research Council Canada-n R C Research Press | |
| dc.relation | Canadian Journal of Physiology and Pharmacology | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | renal tubules | |
| dc.subject | microperfusion | |
| dc.subject | pH | |
| dc.subject | maxi K channels | |
| dc.subject | Na(+)/H(+) | |
| dc.subject | H-ATPase | |
| dc.title | Effect of uroguanylin on potassium and bicarbonate transport in rat renal tubules | |
| dc.type | Otro | |
